DNA Manipulation Techniques in Recombinant DNA Technology

DNA manipulation plays a crucial role in recombinant DNA technology, where pure DNA molecules are manipulated to create recombinant DNA molecules. Techniques like cutting and joining specific DNA segments using vectors are key steps in this process. Enzymes such as nucleases, ligases, polymerases, and modifying enzymes are employed to carry out DNA manipulation within a test tube. These manipulations are not only vital in gene cloning experiments but also in DNA biochemistry, gene structure studies, and gene expression control research. The importance of purified enzymes in genetic engineering, particularly in gene cloning procedures involving restriction endonucleases and ligases, is highlighted.

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