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Cytoskeleton

Cytoskeleton

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Cytoskeleton

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  1. Cytoskeleton

  2. Cytoskeleton: components

  3. Actin structure

  4. Actin treadmilling

  5. Actin-based motility

  6. Pollard and Borisy, 2003

  7. Actin branching

  8. The ARP complex Regulates Actin Polymerization Arp2 and Arp3 cannot form polymers due to differences in their minus ends Figure 16-28 part 1 of 2. Molecular Biology of the Cell, 4th Edition

  9. WASP/WAVE proteins regulate ARP2/3 complex ARP2/3 ARP2/3 ARP2/3 ARP2/3 G-actin WASP/SCAR

  10. R R ARP2/3 ARP2/3 ARP2/3 ARP2/3 R G-actin R R SCAR SCAR

  11. ARP2/3 G-actin R SCAR

  12. NAP PIR ABI BRK R

  13. Brk is part of SCAR complex NO BRICK BRICK Wild type brk

  14. NO ARP2/3 ARP2/3 Wild type arp2

  15. SCAR activates ARP2/3 in vitro: ? NO SCAR SCAR Wild type SCAR?

  16. Mutant analysis ? NAP ARP2/3 SCAR PIR ABI BRK

  17. Activatory mode ? ARP2/3 NAP SCAR PIR ABI BRK

  18. There are different ways to knock out gene function: • start with mutant - analyze phenotype • introduce RNAi-analyze phenotype NAP ? SCAR PIR ABI BRK

  19. Another way to study gene function - analyze it’s expression NAP SCAR PIR ABI BRK BRK

  20. Another way to study gene function - analyze it’s expression BRK