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  1. [Trypsin] (mg/ml) [Trypsin] (mg/ml) 1000 1000 100 100 0.1 0.1 10 10 0 0 1 1 WT DF508 Mature Mature Immature Immature DF508/ PolyValine PolyValine Mature Mature Immature Immature DF508/ S728A S728A Mature Mature Immature Immature DF508/ E725K/E726K E725K/E726K Mature Mature Immature Immature DF508/ T717A/E725K T717A/E725K Mature Mature Immature Immature