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Evaluation of Relative Infectivity and MFI in Different Cell Lines Using hA3G Constructs

This study investigates the relative infectivity of modified hA3G constructs across various cell lines, including human, rabbit, ferret, hamster, rat, and mouse models. We present multiple flow cytometry results, showing mean fluorescence intensity (MFI) differences among constructs. The data includes MFI readings for both mock and hA3G conditions, allowing for a comparative analysis of infectivity. Figures S1 to S5 provide detailed observations, including sequence information and experimental methodologies related to the constructs used.

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Evaluation of Relative Infectivity and MFI in Different Cell Lines Using hA3G Constructs

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  1. 101 NL-luc wt NL-luc vif 100 Relative infectivity 10-1 10-2 Mock pcDNA-hA3G pcDNA-rabbit A1 Figure S1

  2. Figure S2 Mock hA3G Nega MFI: 98.0 MFI: 25.7 MFI: 29.1 Human A1 Ferret A1 Rabbit A1 MFI: 105.0 MFI: 103.2 MFI: 39.0 SSC-H Hamster A1 Rat A1 Mouse A1 MFI: 74.0 MFI: 75.8 MFI: 74.4 GFP

  3. Figure S3 A B Mock hA3G Nega Mock hA3G Nega MFI: 259.3 MFI: 18.2 MFI: 17.3 MFI: 259.3 MFI: 18.2 MFI: 17.3 HumanA1 Ferret A1 Rabbit A1 SSC-H Rabbit A1 MFI: 114.1 MFI: 184.4 MFI: 18.2 SSC-H WT E63A E63Q MFI: 18.2 MFI: 35.6 MFI: 56.3 Hamster A1 Rat A1 Mouse A1 MFI: 17.3 MFI: 22.5 MFI: 47.8 GFP GFP

  4. Figure S4 2137 caga gcagaccaga gccaacagcc 2161 ccaccagaag agagcuucag guuuggggaa gagacaacaa cucccucuca gaagcaggag   1 2221 ccgauagaca aggaacugua uccuuuagcu ucccucagau cacucuuugg cagcgacccc 31147 2281 ucgucacaau aaagauaggg gggcaauuaa aggaagcucu auuagauaca ggagcagaug 21514 2341 auacaguauu agaagaaaug aauuugccag gaagauggaa accaaaaaug auagggggaa 1 2401 uuggagguuu uaucaaagua agacaguaug aucagauacu cauagaaauc ugcggacaua   27615135 2461 aagcuauagg uacaguauua guaggaccua caccugucaa cauaauugga agaaaucugu 12251 2521 ugacucagau uggcugcacu uuaa

  5. Caco-2 293T HeLa ACF 1312F/1596R ACF 1363F/1512R GAPDH Figure S5 RT + - + - + -

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