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Introduction to Peptide Structure Determination

Amino Acid Analysis. Amino Acid Analysis. Acid-hydrolysis of the peptide (6 M HCl, 24 hr) gives a mixture of amino acids.The mixture is separated by ion-exchange chromatography.Amino acids are detected using ninhydrin.Automated method; requires only 10-5 to 10-7 g of peptide.. Partial Hyd

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Introduction to Peptide Structure Determination

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    1. Introduction to Peptide Structure Determination

    2. Amino Acid Analysis

    3. Amino Acid Analysis Acid-hydrolysis of the peptide (6 M HCl, 24 hr) gives a mixture of amino acids. The mixture is separated by ion-exchange chromatography. Amino acids are detected using ninhydrin. Automated method; requires only 10-5 to 10-7 g of peptide.

    4. Partial Hydrolysis of Proteins

    5. Partial Hydrolysis of Peptides and Proteins Acid-hydrolysis of the peptide cleaves all of the peptide bonds. Cleaving some, but not all, of the peptide bonds gives smaller fragments. These smaller fragments are then separated and the amino acids present in each fragment determined. Enzyme-catalyzed cleavage is the preferred method for partial hydrolysis.

    6. Carboxypeptidase

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