Objectives

1. The effect of Eicosapentaenoic acid (omega-3) on the expression of HAS2 in cumulus cells on mice Behnaz Valipour, Leila Roshangar, Jafar Soleimani Rad , Hajar Shafayi Department of Anatomical Science , Faculty of Medicine , Tabriz University of Medical Science Methods Objectives Omega-3 fatty acids can modify gene and protein expression through altering the function of transcription factors controlling gene expression. Cumulus expansion ,is a critical process for normal oocyte development, ovulation, and fertilization, this event needs to the synthesis of hyaluronic acid and the production of that is controlled by the enzyme hyaluronic acid synthase 2 (HAS2). The aim of this study was to determine if Omega-3 can affect on the expression of HAS2 in Cumulus cells and how was its outcome in the in vitro fertilization for this purpose, 30 female mice were superovulated with hMG and hCG as IP injection. for the collection of cumulus- oocyte complexes(COCs) mice were killed by cervical dislocation and COCs were collected by flashing method .COCs were cultured in2cc universal medium supplemented with 20 גּ omega-3 and incubated for 4-6 hrs. surrounding cumulus cells were removed mechanically by using 20גּ hyaluronidase.the cumulus cells was stored at- 80 ˚C until real-time PCR.PCR was performed by using a master mix containing CYBR green. The expansion level of mRNA HAS2 was evaluated by real-time PCR . In other group for the IVF COCs and sperms from male mice from the caudal epididymis by incubating the pieces of epididymis in37˚C oven. The sperm were added to the medium drops containing 50 COCs in each group and embryo formation was considered as fertility. The number of embryos in each group was counted and compared with each other by using ANOVA. Results The result showed that the percent of embryo formation and the level of expansion of HAS2 in experimental group comparing with control were significantly increased. Conclusions References The result indicate that omega-3 could improve Cumulus expansion through increasing the amount of expansion of HAS2. Cleavage in control group after 48 hours Keywords:omega-3 ,HAS2,cumulus cells