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Impact of Phage Treatment on Tomato Plant Wilting Caused by R. solanacearum Infection

This study investigates the effects of pre-treating one-month-old tomato plants with various phage mixtures on the wilting symptoms caused by R. solanacearum. The plants were treated with tap water (control), a mixture of three phages (ΦRSA1, ΦRSB1, and ΦRSL1), or a mixture of two phages (ΦRSA1 and ΦRSB1) before inoculation with R. solanacearum cells. Wilting symptoms were assessed on a scale from 0 (no symptoms) to 5 (plant death), providing insights into the potential of phage therapy for managing bacterial wilt in tomatoes.

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Impact of Phage Treatment on Tomato Plant Wilting Caused by R. solanacearum Infection

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  1. FIG. S1. Effects of pre-treatment of tomato plants with a phage mixture on wilting caused by R. solanacearum. One-month old tomato plants (2023 cm in height) pretreated with tap water (A, control), a mixture of three phages (RSA1, RSB1, and RSL1) (B), or a mixture of two phages (RSA1 and RSB1) were inoculated with R. solanacearum cells as described in the Materials and Methods. Wilting symptoms were graded from 05; 0, no symptom; 1, only one petiole was wilting; 2, 23 petioles were wilting; 3, all but 23 petioles were wilting; 4, all petioles were wilting; 5, the plant died.

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