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Investigating AML1-ETO Expression and Its Effect in HSPC Using Cre-LoxP System

This study explores the expression of AML1-ETO in hematopoietic stem/progenitor cells (HSPCs) using a pMSCV vector system with IRES.GFP for dual expression. We utilize a Cre-LoxP approach to conditionally control the expression of AML1-ETO, tracking the effects on HSPC behavior. Probing mRNA levels reveals insights into relative expression changes among experimental groups. Various constructs, including Cre-YFP and HA-AML1-ETO, are analyzed to clarify their roles in HSPC differentiation and proliferation. Results contribute to understanding AML1-ETO-driven leukemogenesis.

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Investigating AML1-ETO Expression and Its Effect in HSPC Using Cre-LoxP System

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  1. Figure S1 A HSPC-AE pMSCV HA AML1-ETO IRES GFP AE HSPC-control + + - - AEfl/fl - - + + Cre-YFP - + - + AEfl/fl YFP + - + - pMSCV IRES GFP Cre-YFP YFP HA-AML1-ETO Tubulin loxP AML1-ETO IRES GFP loxP B C - 98 kDa Cre IRES YFP Input Unbound Anti-HA IP - 55 kDa mRNA relative expression loxP IRES YFP loxP

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