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Fluorescence Microscopy

Fluorescence Microscopy. Test your prediction skills. Hoechst stain. The structure of these stains causes them to slide in between the bases of DNA and fluoresces blue. What structure of the cell would you predict would be stained?. Human cheek cell. We’ll come back to the green.

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Fluorescence Microscopy

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  1. Fluorescence Microscopy Test your prediction skills.

  2. Hoechst stain • The structure of these stains causes them to slide in between the bases of DNA and fluoresces blue. • What structure of the cell would you predict would be stained?

  3. Human cheek cell We’ll come back to the green.

  4. Connective tissue cells – fibroblasts We’ll come back to the red and green.

  5. Bull sperm The heads of sperm are filled completely with this structure. We’ll come back to the green.

  6. DiOC6associates with lipids and produces a green color What subcellular structures are lipid based?

  7. Human cheek cell

  8. Ovary cells from Chinese Hamster

  9. Oxidation revealer red Concentrated in the organelle that uses oxygen to generate ATP

  10. Connective tissue cells - fibroblasts

  11. Oxidation revealer green Same characteristics as oxidation revealer red, but a different color fluorescence

  12. oxidation revealing greensame structure, highly elongated shape Fathead minnow cell

  13. The tails of sperm are packed with these structures.

  14. acid loving red Accumulates in organelles with an acid pH, useful for breaking down wastes

  15. Canine kidney cells We’ll come back to the green, which is a different dye from the one you’ve seen already.

  16. BODIPY ceramide(green) Ceramides are molecules taken up and metabolized by the organelle responsible for for transporting lipids and proteins to the cell membrane

  17. Canine kidney cells

  18. Fibroblast cells

  19. Phalloidin green Binds to filamentous actin

  20. Phalloidin red Binds to filamentous actin

  21. Bovine pulmonary artery endothelial cells – the cells lining the interior of the major vessel bringing blood into the lungs. In this picture, the green is a microtubule stain. The red is phalloidin.

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