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  1. Gene correction in trans:a model for gene therapy gfp- Luther Davis Jason Cummings Nancy Maizels

  2. Gene correction in trans:a model for gene therapy GFP+ Luther Davis Jason Cummings Nancy Maizels

  3. nuclease Gene correction in trans Cut sites eGFP Poly-lacO Puro trGFP eGFP Poly-lacO Puro

  4. nuclease Gene correction in trans Cut sites eGFP Poly-lacO Puro trGFP eGFP Poly-lacO Puro

  5. I-AniIY2 promotes correction in trans + donor + I-SceI GFP + donor + AniY2 FL2-H

  6. I-AniIY2 “nickase” promotes correction in trans + donor + AniY2 GFP + donor + AniY2-nick FL2-H

  7. nuclease tethered factor Modulation of gene correction in trans: tethering factors at recipient Cut sites eGFP Poly-lacO Puro trGFP eGFP Poly-lacO Puro

  8. Recipient chromatin structure regulates gene correction in trans + LacI GFP + HP1-LacI * Average of two experiments FL2-H

  9. tethered factor trGFP Modulation of gene correction in trans: tethering factors at donor Cut sites eGFP Puro nuclease Poly-lacO eGFP Puro

  10. HP1 tethered at donor blocks correction in trans 104 103 104 0.24% 102 103 101 102 100 101 100 101 102 103 104 100 100 101 102 103 104 0.22% 103 LacO donor +ISceI LacO donor +ISceI +LacI 102 101 100 100 101 102 103 104 104 0.005% 40-fold decrease in recombination LacO donor +ISceI +HP1-LacI GFP FL2 Height Jason Cummings