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Investigating the Role of GFP-ERα in Proteasome Function and Hormonal Response

This study explores the interactions of GFP-ERα with the proteasome in the presence of various treatments, including estrogen (E2) and inhibitors like ICI and RU58. The experimental setup utilizes vehicle controls and investigates the effects of ALLN on ERα induction. Through immunoblotting and relative fold induction analysis, we aim to elucidate the mechanisms underlying ERα's role in cellular responses to hormonal stimuli, with GAPDH serving as a loading control. Results may reveal insights into estrogen signaling pathways and proteasome modulation.

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Investigating the Role of GFP-ERα in Proteasome Function and Hormonal Response

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  1. -- -- -- -- LMB ALLN ALLN ALLN ALLN LMB LMB LMB A B GFP-ERa proteasome merged Nu a b c vehicle f e d E2 Cyt +E2 500 nm 100 nm C D E2 ICI RU58 GFP-ERa proteasome merged vehicle c a b 1 2 3 4 5 6 7 8 9 10 11 12 GFP-ERa ICI * ERa e d f GAPDH ICI + ALLN * - unspecific band h g i RU58 Relative ERa fold induction j k l RU58 + ALLN EtOH EtOH E2 ICI RU58 E2 ICI RU58 GFP-ERa ERa

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