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  1. 10.0 9.0 8.0 7.0 6.0 Ratio 5.0 4.0 3.0 2.0 1.0 0.0 FIGURE 1A Abbott Architect i2000 (I) Abbott Architect i2000 (I) 10.0 9.0 8.0 7.0 6.0 Ratio 5.0 4.0 3.0 2.0 1.0 0.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  2. 10.0 10.0 9.0 9.0 8.0 8.0 7.0 7.0 6.0 6.0 Ratio Ratio 5.0 5.0 4.0 4.0 3.0 3.0 2.0 2.0 1.0 1.0 0.0 0.0 Abbott Architect i2000 (II) Abbott Architect i2000 (II) 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  3. Beckman Coulter Access Beckman Coulter Access 10.0 10.0 9.0 9.0 8.0 8.0 7.0 7.0 6.0 6.0 Ratio Ratio 5.0 5.0 4.0 4.0 3.0 3.0 2.0 2.0 1.0 1.0 0.0 0.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  4. Siemens Coat a Count DPC Siemens Coat a Count DPC 10.0 10.0 9.0 9.0 8.0 8.0 7.0 7.0 6.0 6.0 Ratio Ratio 5.0 5.0 4.0 4.0 3.0 3.0 2.0 2.0 1.0 1.0 0.0 0.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  5. Siemens ADVIA Centaur Siemens ADVIA Centaur 10.0 10.0 9.0 9.0 8.0 8.0 7.0 7.0 6.0 6.0 Ratio Ratio 5.0 5.0 4.0 4.0 3.0 3.0 2.0 2.0 1.0 1.0 0.0 0.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  6. 10.0 10.0 9.0 9.0 8.0 8.0 7.0 7.0 6.0 6.0 Ratio Ratio 5.0 5.0 4.0 4.0 3.0 3.0 2.0 2.0 1.0 1.0 0.0 0.0 Siemens Immulite 2000 Siemens Immulite 2000 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  7. Roche Cobas (II) Roche Cobas (II) 10.0 10.0 9.0 9.0 8.0 8.0 7.0 7.0 6.0 6.0 Ratio Ratio 5.0 5.0 4.0 4.0 3.0 3.0 2.0 2.0 1.0 1.0 0.0 0.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  8. FIGURE 1B Abbott Architect i2000 (I) Abbott Architect i2000 (I) 2.0 2.0 1.8 1.8 1.6 1.6 1.4 1.4 1.2 1.2 Ratio Ratio 1.0 1.0 0.8 0.8 0.6 0.6 0.4 0.4 0.2 0.2 0.0 0.0 4.0 8.0 12.0 16.0 24.0 28.0 32.0 36.0 40.0 20.0 4.0 8.0 12.0 16.0 20.0 24.0 28.0 32.0 36.0 40.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  9. 2.0 2.0 1.8 1.8 1.6 1.6 1.4 1.4 1.2 1.2 1.0 1.0 0.8 0.8 0.6 0.6 0.4 0.4 0.2 0.2 0.0 0.0 Abbott Architect i2000 (II) Abbott Architect i2000 (II) Ratio Ratio 4.0 8.0 12.0 16.0 20.0 24.0 28.0 32.0 36.0 40.0 4.0 8.0 12.0 16.0 20.0 24.0 28.0 32.0 36.0 40.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  10. 2.0 1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Beckman Coulter Access Beckman Coulter Access 2.0 1.8 1.6 1.4 1.2 Ratio Ratio 1.0 0.8 0.6 0.4 0.2 0.0 4.0 8.0 12.0 16.0 20.0 24.0 28.0 32.0 36.0 40.0 4.0 8.0 12.0 16.0 20.0 24.0 28.0 32.0 36.0 40.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  11. Siemens Coat a Count DPC Siemens Coat a Count DPC 2.0 2.0 1.8 1.8 1.6 1.6 1.4 1.4 1.2 1.2 Ratio Ratio 1.0 1.0 0.8 0.8 0.6 0.6 0.4 0.4 0.2 0.2 0.0 0.0 4.0 8.0 12.0 16.0 20.0 24.0 28.0 32.0 36.0 40.0 4.0 8.0 12.0 16.0 20.0 24.0 28.0 32.0 36.0 40.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  12. Siemens ADVIA Centaur Siemens ADVIA Centaur 2.0 2.0 1.8 1.8 1.6 1.6 1.4 1.4 1.2 1.2 Ratio Ratio 1.0 1.0 0.8 0.8 0.6 0.6 0.4 0.4 0.2 0.2 0.0 0.0 4.0 8.0 12.0 16.0 20.0 24.0 28.0 32.0 36.0 40.0 4.0 8.0 12.0 16.0 20.0 24.0 28.0 32.0 36.0 40.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)

  13. 2.0 2.0 1.8 1.8 1.6 1.6 1.4 1.4 1.2 1.2 1.0 1.0 0.8 0.8 0.6 0.6 0.4 0.4 0.2 0.2 0.0 0.0 4.0 4.0 8.0 8.0 12.0 12.0 16.0 16.0 20.0 20.0 24.0 24.0 28.0 28.0 32.0 32.0 36.0 36.0 40.0 40.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L) Siemens Immulite 2000 Siemens Immulite 2000 Ratio Ratio

  14. Roche Cobas (II) Roche Cobas (II) 2.0 2.0 1.8 1.8 1.6 1.6 1.4 1.4 1.2 1.2 Ratio Ratio 1.0 1.0 0.8 0.8 0.6 0.6 0.4 0.4 0.2 0.2 0.0 0.0 4.0 8.0 12.0 16.0 20.0 24.0 28.0 32.0 36.0 40.0 4.0 8.0 12.0 16.0 20.0 24.0 28.0 32.0 36.0 40.0 ID-LC-MS/MS (nmol/L) ID-LC-MS/MS (nmol/L)