Effect of Antimycin A on NPQ and Photoinhibition in Wild Type and pgr5 Mutant Leaf Discs
This study investigates the impact of antimycin A (AA) on non-photochemical quenching (NPQ) and photoinhibition in leaf discs from both wild type and pgr5 mutant plants. The discs were exposed to light at 1000 µmol photons m-2 s-1, with comparisons made between treatments involving AA and chloramphenicol (Cm). Maximum quantum yield of PSII (Fv/Fm) was measured post-incubation. Results demonstrate the role of AA in inducing NPQ and its influence on photoinhibition, highlighting implications for plant light response mechanisms.
Effect of Antimycin A on NPQ and Photoinhibition in Wild Type and pgr5 Mutant Leaf Discs
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Presentation Transcript
Light intensity (µmol m-2 s-1) 1000 0 AA - + wt pgr5 - + A 3.0 AA 2.5 - + wt - + pgr5 2.0 NPQ 1.5 1.0 0.5 0.0 0 5 10 15 20 25 Duration of incubation (min) B 0.9 Control (-Cm) +Cm 0.8 0.7 0.6 0.5 Fv/Fm 0.4 0.3 0.2 0.1 0.0 0 1 2 3 0 1 2 3 Duration of incubation in light (h) Supplemental Figure 1. Effect of antimycin A on NPQ and photoinhibition. Leaf discs (78.5 mm2) from the wild type and the pgr5 mutant were exposed to light at 1000 µmol photons m-2 s-1 in the presence of 250 µM antimycin A (AA) or in its absence. A, Induction of NPQ. B, Leaf discs were exposed to light in the presence of 1 mM chloramphenicol (Cm) or in its absence (Control). The maximum quantum yield of PSII (Fv/Fm) was measured after dark incubation for 15 min. In A and B, the values are mean ± SD (bars) of results from three independent experiments.
