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Analysis of L-Arabinose and L-Rhamnose Concentrations in Mice Serum and Intestinal Contents

This study employs GC-MS analysis to determine the concentrations of L-arabinose and L-rhamnose in the serum and intestinal contents of mice. Following intraperitoneal (i.p.) injection of inducers, serum samples were collected and analyzed. In a separate experiment, induction via oral administration involved providing drinking water supplemented with these sugars over a 24-hour period. The analysis offers insights into the absorption and metabolism of these sugars, with serum and intestinal samples collected from multiple individual mice and pooled for study.

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Analysis of L-Arabinose and L-Rhamnose Concentrations in Mice Serum and Intestinal Contents

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  1. 4 4 3 3 sugar concentration log ng/µl serum or ng/µg sugar concentration log ng/µl serum or ng/µg 2 2 1 1 0 0 Suppl. Fig. 2 A B C i.p. oral L-arabinose oral L-rhamnose 4 Serum Serum L-arabinose Cecum Cecum L-rhamnose SI SI 3 LI LI sugar concentration log ng/µl serum 2 1 0 0 10 20 30 40 50 0 2 4 6 8 10 0 10 20 30 40 50 Time (h) Time (h) Time (h) Supplementary Figure 2. Determination of L-arabinose and L-rhamnose concentrations in serum and intestinal contents of mice. (A) Intraperitoneal (i.p.) injection of inducers was followed by GC-MS analysis of serum. (B,C) Inducer administration via the oral route was followed by GC-MS analysis of serum and intestinal contents of mice. Here, mice received drinking water with respective sugars for 24 hrs. The dotted line indicates the exchange of supplemented water against normal water. Serum samples were pools of at least three mice and intestinal samples were obtained from individual mice.

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