1 / 20

PROTEINS

PTT 202: ORGANIC CHEMISTRY FOR BIOTECHNOLOGY. PROTEINS. PREPARED BY: NOR HELYA IMAN KAMALUDIN helya@unimap.edu.my. Introduction of Proteins. Separation of Proteins. Precipitation. Electrophoresis. Technical aspects of electrophoresis. 1. Supporting medium.

clare
Télécharger la présentation

PROTEINS

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PTT 202: ORGANIC CHEMISTRY FOR BIOTECHNOLOGY PROTEINS PREPARED BY: NOR HELYA IMAN KAMALUDIN helya@unimap.edu.my

  2. Introduction of Proteins

  3. Separation of Proteins

  4. Precipitation

  5. Electrophoresis

  6. Technical aspects of electrophoresis

  7. 1. Supporting medium

  8. 2. Iso-electric focusing technique

  9. 3. Buffer pH

  10. 4. Buffer concentration

  11. 5. The methods of capillary electophoresis performed

  12. Quantitative aspects for electrophoresis

  13. 1. Semi-quantitative method

  14. 2. Alternative quantitative method

  15. Immunological methods

  16. The steps of immunoblotting

  17. The steps of immunoblotting (cont)

  18. Immunoaffinity purification

  19. Chromatographic methods

  20. THANK YOU..

More Related