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Advanced Plasmid Constructs for pduP Gene and Protein Expression in E. coli

This study presents a series of innovative plasmid constructs designed for the efficient expression of the pduP gene in E. coli. Utilizing vectors such as pET28a and pGEM-T Easy, we explore the functionality of various promoter systems, including T7 and PlacZ, alongside co-expression of auxiliary genes such as dhaT and orf13/14. The plasmids allow for tailored expression profiles to optimize recombinant protein production. Supplementary data, including experimental figures, provide detailed insights for researchers in synthetic biology and genetic engineering.

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Advanced Plasmid Constructs for pduP Gene and Protein Expression in E. coli

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  1. a X N X N H H pduP T7 pET28a pGEM-T Easy-pduP (N+X) N X pduP H T7 pET-pduP b Xb B Xb X B X pduP P pGEM-T Easy-PlacZ pGEM-T Easy-pduP (Xb+B) Xb X B X X pduP P dhaT P orf13 orf14 P pVOT pGEM-T Easy-P-pduP (X) X X P dhaT P pduP P orf13 orf14 pVOTLp Supplementary Fig 1

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