1 / 29

RECOMBINANT DNA TECHNOLOGY

RECOMBINANT DNA TECHNOLOGY. PART 2. Topics. Genomic libraries Id a specific clone or sequence within a library Transgenic plants Transgenic animals Timely topics. Genomic library contains all of the organism’s genome. Probe design. Bacterial clones and finding desired clone.

donny
Télécharger la présentation

RECOMBINANT DNA TECHNOLOGY

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. RECOMBINANT DNA TECHNOLOGY PART 2

  2. Topics • Genomic libraries • Id a specific clone or sequence within a library • Transgenic plants • Transgenic animals • Timely topics

  3. Genomic library contains all of the organism’s genome Probe design

  4. Bacterial clones and finding desired clone Probe design

  5. A Final about vectors : There are many types  • Small vectors • Large vectors • Expression vectors • Viral vectors

  6. Transgenic Mice

  7. transgene Non-homologous recombination LinearDNA

  8. Knockout Mice

  9. G418 gancyclovir Homozygous knockout Phenotype of the knockout mouse gives information on protein function

  10. Applications of DNA technology • Agriculture • Drug design • Genetic testing • Gene therapy

  11. Genetic Modified Plants: “GM” Tobacco with luciferase gene from firefly Transgenic plant

  12. Tumor Inducing Plasmid

  13. Model for making genetically modified (GM) plants

  14. GM crops • Bt toxin genes (Bacillus thuringensis)

  15. Bt Toxin Cry toxins and cry genes: damages gut of larvae

  16. GM Plants • Roundup  ready plants: herbicide resistant • Herbicide known as: Glyphosate

  17. Glyphosate • Enzyme inhibitor • Prevents amino acid synthesis • Prevents weed growth • Environmental concerns

  18. Antisense Technology • 5´C A U G   3´     mRNA3´   G U A C   5´     Antisense RNA

  19. Anti-Sense Technology &FLAVR SAVRTM Tomato • Anti-sense DNA technology inhibits polygalacturonase (PG)

  20. The Flavr-Savr Tomato • Ripens on the vine • Preserves firmness and flavor • Uses anti-sense technology

  21. Cloning of a whole organism: Reproductive Cloning

  22. SHEEP CELLS 54 27 Somatic Cell Germ Cell For ex. Mammary cell ovum

  23. How to process begins: 54 27 54 discarded Somatic Cell Germ Cell For ex. Mammary cell ovum

  24. Now the germ cell contains 54 chromosomes! • The germ is stimulated “electrically”. • Cell division in some instances begins. • Individual is identical to host “DNA” donor. • Aging affects? “Was Dolly a sheep in lamb’s clothing?”

  25. Somatic Gene Therapy

  26. Successes • SCIDS (Severe Combined Immunodeficiency Disease) • Head and neck cancers

  27. But also failures and controversy

More Related