1 / 8

Investigating Akt Phosphorylation in HL-60 Cells with fMLP and Wortmannin

This study explores the effects of fMLP and wortmannin on Akt phosphorylation in HL-60 cells, utilizing various techniques including PH-Akt-GFP analysis and PIP3 antibody detection. Key findings are illustrated in multiple figures, showing the relative levels of T308P-Akt and S473P-Akt under different conditions. The results reveal significant insights into the signaling pathways modulated by fMLP and the inhibition by wortmannin, emphasizing the role of these agents in cell signaling and immune responses.

laurie
Télécharger la présentation

Investigating Akt Phosphorylation in HL-60 Cells with fMLP and Wortmannin

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. e a b unstimulated +fMLP T308P-Akt S473P-Akt Akt c d (min) 0 0.5 1 1.5 2 4 + fMLP (100 nM) PH-Akt-GFP Anti-PIP3 antibody Figure 1a-e, supplement

  2. f (min) 0 1 1.5 0.5 2 4 + fMLP (100 nM) Figure 1f, supplement

  3. a Overlay DiD PH-Akt-GFP b Ratio (PH-Akt-GFP/DiD) Fig. 2a-b, supplement

  4. c Fig. 2c, supplement

  5. a HL-60 + 50 nM wort Figure 3a, supp

  6. d b 120 100 80 Relative level of T308P-Akt (%) 60 + 200 nM wort 40 c 20 0 120 T308P-Akt 100 80 S473P-Akt Relative level of S473P-Akt (%) 60 40 Akt 20 0 1 2 3 4 5 6 7 HL-60 + 50 mM LY + 200 mM LY + 400 mM LY HL-60 + 20 nM wort + 50 nM wort HL-60 +fMLP + 200 nM wort + 50 mM LY + 200 mM LY + 400 mM LY + 20 nM wort + 50 nM wort + 200 nM wort Figure 3b-d, supplement

  7. a HL-60 + wort Paths 10 min 6 min 2 min c b + fMLP 150 HL-60 100 50 3 0 2 4 1 HL-60 HL-60 + wort + 50 nM wort + 200 nM wort Figure 4, supplement

  8. b a 4000 Cell Number 2000 0 0 0.1 1 10 100 Human blood neutrophils Log [fMLP] (nM) c + 30 nM wortmannin PLB-985 + 200 mM LY 294002 Figure 5, supplement

More Related