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Lab 2: Protists. Protists !. Lab Goals. Identify the three domains of life and the main characteristics of each Define protists and know why the group “ Protista ” is not an appropriate taxon Be able to recognize the main supergroups , subgroups, and kingdoms of protists
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Lab Goals • Identify the three domains of life and the main characteristics of each • Define protistsand know why the group “Protista” is not an appropriate taxon • Be able to recognize the main supergroups, subgroups, and kingdoms of protists • Practice microscope skills
Separation based on rRNA sequences. Kingdom Monera is comprised of 2 separate monophyletic groups: Eubacteria Archaebacteria 5 Kingdoms or 6 Kingdoms?
Phylogenetic tree of Domain Eukarya (“Protista” in yellow) Why is Protista not a good kingdom?
Solution: Domain (Eukarya) Supergroup Subgroup Kingdom Phylum Etc.
Bacteria Domain Contain Peptidoglycan in Cell Walls. Archaea Domain Contain Histones associated with DNA. Likely share more Recent Common Ancestor with Eukaryotes than with Bacteria. Live EVERYWHERE! The Prokaryotic Domains: Bacteria & Archaea
Bacteria No nucleus Peptidoglycan in cell walls Archaea No nucleus Histones associated with DNA. Three Domains of Life • Eukarya • Membrane-bound nucleus and organelles • Histones associated with DNA • Unicellular or Multicellular More closely related to each other than to Bacteria!
What is a protist? • Protist=Eukaryote not classified as a plant, animal, or fungi • NOT a monophyletic group!
Why are protists important? • Disease • “Beaver Fever” (Giardia) • Red Tide • Malaria • Toxoplasmosis • African Sleeping Sickness
MICROSCOPES! (Appendix B, p.213-218) • Start at 4x or 10x • See manual for preparing slides • Save any really good slides for others • Will save everyone time • Clean the slides when you are done