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Supplementary Figure S4. Martín et al.

B. A. C.

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Supplementary Figure S4. Martín et al.

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  1. B A C Supplementary Figure S4. Purification of the inducer molecule through gel filtration chromatography (Sephadex G-10) and HPLC. A) Qualitative bioassay of inducer activity carried out with different fractions eluted from the gel filtration column. B)HPLC analysis of the bioactive fractions obtained through gel filtration chromatography. Samples were derivatizated with FMOC and detection was achieved at 254 nm (see Materials and Methods). C) Qualitative bioassay of inducer activity carried out with the five fractions collected from the HPLC analysis shown in B). Supplementary Figure S4. Martín et al.

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