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Program in NHP Neuroimaging

Program in NHP Neuroimaging. Calum Avison. Overview. Rationale Biophysics Biology / behavior Facilities / Capabilities Imaging Support Equipment Staff Projects DTI Tractography Validation Rhesus (3T) - neurodevelopmental consequences of cocaine exposure in utero

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Program in NHP Neuroimaging

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  1. Program in NHP Neuroimaging Calum Avison

  2. Overview • Rationale • Biophysics • Biology / behavior • Facilities / Capabilities • Imaging • Support Equipment • Staff • Projects • DTI Tractography Validation • Rhesus (3T) - neurodevelopmental consequences of cocaine exposure in utero • Squirrel monkey (9.4T) - neural correlates of tactile illusion / correlation with optical imaging and electrophysiology

  3. Structural Homology? Functional Homology? NHP Rationale - Biology

  4. Project 1 - Prenatal Cocaine Exposure (PNCE)

  5. Prenatal Cocaine Exposure in Rhesus • Developmental neurobiology • PNCE  behavioral/cognitive impairment? • Structure • Functional circuitry • Pre-/post-synaptic defects in neurotransmission • Methodology • mPET mapping of receptors, neurotransmitter release • High resolution structural MRI + VBM • High resolution DTI • fMRI responses to pre-/post-synaptic agents • Co-registration

  6. Co-registered (ABA) PET/MR images of Rhesus Monkey T1-weighted MR image acquired on a human 3T scanner Summed image from a 3-hr acquisition after 4.9 mCi injection of the D2 receptor ligand [F-18]fallypride

  7. p_2_22 p_2_31 p_2_27 p_2_17 HARDI / FORECAST - NHP (92 directions) 1.5 mm isotropic 2ch FLEX-M coil SENSE, R=1.5

  8. DTI Tractography

  9. p_2_31 p_2_27 p_2_22 p_2_17 HARDI / FORECAST - NHP (92 directions) 0.8 mm isotropic 2ch FLEX-M coil SENSE

  10. Implementation on Intera Achieva 3T • Humans - 2 mm isotropic; 32, 92 directions • Neonatal development • Normal and abnormal • Developmental abnormalities • Correlation with ss-fMRI (structure begets function) • NHPs - 0.8 mm isotropic; 92 directions • Validation of HAR diffusion methods • Structural homology (y/n?) with humans • y  Model of human brain • n  Brain evolution • Effects of prenatal cocaine exposure

  11. HARDI / FORECAST - Human (92 directions) h_03

  12. HARDI / FORECAST Human vs NHP (92 directions) p_2_17 h_03

  13. HARDI / FORECAST Human vs NHP (92 directions) p_2_22 h_09

  14. HARDI / FORECAST Human vs NHP (92 directions) p_2_25 h_10

  15. NHP at 3T • 2ch FLEX-M coil • SENSE

  16. NHP at 3T

  17. NHP at 3T

  18. NHP at 3T

  19. Coregistration (Overlay) functional 2D anatomic Coregistration using gyri information to match 2D and 3D Draw Volumes-of-interest • Look at timecourse information for each VOI • - Statistics: GLM, ROI analysis

  20. Volumes-of-interest supragenual anterior cingulate cortex (ACC1) Caudate head (Divided into RH and LH) Subgenual anterior cingulate cortex (ACC2) Putamen (Divided into RH and LH)

  21. BrainVoyager Capability General Linear Model Graph and Table of beta weight values Can apply statistics and obtain timecourse of only significantly activated voxels. (above: TC of significantly activated voxels of all six determined volumes-of-interest. 1.0 mg Cocaine administered at volume 200.)

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