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Expression Analysis of SMYD2 and ATF3 mRNA in ESCC Cell Lines Compared to Normal Esophagus

This study investigates the relative mRNA expression of SMYD2 and ATF3 in esophageal squamous cell carcinoma (ESCC) cell lines compared to normal esophagus tissue. Using densitometry, we quantified SMYD2 protein expression and measured mRNA levels of CENPF. Supplementary Figure S1 presents comparative data on the expression profiles across various cell lines and normal tissue. The findings highlight the dysregulation of these genes in ESCC, contributing to understanding their roles in tumor biology and potential as therapeutic targets.

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Expression Analysis of SMYD2 and ATF3 mRNA in ESCC Cell Lines Compared to Normal Esophagus

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  1. C 200 160 r2 = 0.1975 P = 0.0028 120 Relative SMYD2 protein expression (Arbitrary unit determined by densitometer) 80 40 0 0 1 2 3 4 5 6 Relative SMYD2 mRNA expression (/ normal esophagus) Supplementary Fig. S1 A CENPF mRNA 8 6 Relative mRNA expression (/ normal esophagus) 4 0 1190 2270 2400 1170 1240 1250 1260 1440 2650 960 110 140 180 190 270 410 450 510 520 770 790 850 890 150 170 200 220 350 590 15 30 70 10 11 13 14 1 2 4 5 6 8 9 TE series KYSE series Normal esophagus ESCC cell lines B ATF3 mRNA 4 3 Relative mRNA expression (/ normal esophagus) 2 1 0 2650 1170 1190 1240 1250 1260 1440 2270 2400 410 150 200 220 350 450 110 140 170 180 190 270 510 590 770 520 790 850 960 890 30 70 10 11 13 14 15 2 4 5 8 9 1 6 TE series KYSE series Normal esophagus ESCC cell lines

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