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Comparative Analysis of Gene Expression and Metabolic Activity in Neuroblastoma Cell Lines

This study investigates the relative expression ratios of various genes in neuroblastoma cell lines, DAOY, D458, and Med8A, under conditions of stable overexpression and transient knockdown. Supplementary figures showcase the expression changes in response to pCMX and siCtrl treatments, as well as the metabolic activity measured over several days. The analysis includes specific miRNA subgroups (miR-96, miR-182, miR-183) and evaluates significant differences in metabolic activity. These findings contribute to understanding the mechanisms underlying neuroblastoma pathophysiology.

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Comparative Analysis of Gene Expression and Metabolic Activity in Neuroblastoma Cell Lines

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  1. b Relative expression ratio vs. pCMX DAOY Relative expression ratio vs. pCMX D458 Med Relative expression ratio vs. pCMX Med8A Stable overexpression Supplementary Fig.1 a Relative expression ratio vs. HKsRNAs Native expression

  2. c Relative expression ratio vs. siCtrl DAOY Relative expression ratio vs. siCtrl D458 Med Relative expression ratio vs. siCtrl Med8A Transient knockdown Supplementary Fig.1

  3. Supplementary Fig.2

  4. Supplementary Fig.3

  5. Molecular subgroup miR-96 miR-182 miR-183 Supplementary Fig.4

  6. Supplementary Fig. 5

  7. ** Metabolic activity, ratio vs. Day1 Metabolic activity, ratio vs. Day1 DAOY * Metabolic activity, ratio vs. Day1 Metabolic activity, ratio vs. Day1 Med8A Day Day Day Transient knockdown Stable overexpression Supplementary Fig. 6 *

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