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Role of GRP78 in Regulating IFN Responses in Huh-7 Cells with Dox Treatment

This study investigates the function of GRP78 in Huh-7 cells under different interferon (IFN) treatments with and without Doxorubicin (Dox). Key findings reveal variations in the expression levels of GRP78 and related stress response markers like GADD153 and ATF6. The analysis includes promoter activity assessments, fold changes over various days, and statistical evaluations indicating significant differences (p<0.0005). Supplementary figures illustrate the experimental data, confirming the correlation between GRP78 regulation and IFN signaling pathways.

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Role of GRP78 in Regulating IFN Responses in Huh-7 Cells with Dox Treatment

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  1. A 100 GRP78 90 NS2 80 vector 70 60 50 RLA x 103 40 30 20 10 0 ng 70 90 50 30 10 110 1.75 x 105 Huh-7 cells B 3.5 3.0 2.5. Fold change (Dox+ / Dox-) 2.0 1.5 1.0 0.5 0 Days 3 4 1 2 Tet-on/NS2 Supplementary Fig. 1

  2. A IFN (+) IFN (-) - + IFN core actin B GRP78 GADD153 CMV ATF6 25 40 P < 0.0005 P < 0.0001 140 25 P < 0.0001 P < 0.0001 35 20 120 20 30 100 15 25 15 RLA x 104 80 RLA x 106 RLA x 106 RLA x 104 20 10 60 10 15 40 10 5 5 20 5 0 + - 0 + 0 0 - + - + - IFN IFN IFN IFN Supplementary Fig. 2

  3. A. 300 GRP78 promoter In Huh-7 150 * 35 30 RLA x 104 25 20 15 10 5 0 core null Tuni E1 NS2 E1-p7 E2 B. 75 70 GRP78 promoter In Huh-7.5 + SG 35 ** 30 RLA x103 25 20 15 10 5 0 E1-p7 core E1 E2 null Tuni NS2 -actin 28S RNA Supplementary Fig. 3

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