Relative Migration and Function of shKPNA4 in TNF-α/β Signaling Pathway

S.Figure1 a 1.5 1.5 1.5 1.5 0 h 48 h siKPNA4-2 siNC siKPNA4-1 1.0 1.0 1.0 1.0 Relative migration Relative migration Relative migration Relative migration siNC * * ** ** ** * 0.5 0.5 0.5 0.5 * ** 0 0 0 0 siKPNA4-1 siNC siNC siNC siNC siKPNA4-1 siKPNA4-1 siKPNA4-2 siKPNA4-2 siKPNA4-1 siKPNA4-1 siKPNA4-2 siKPNA4-2 siKPNA4-2 b siKPNA4-2 siNC siKPNA4-1 c 0 h 48 h siNC siKPNA4-1 siKPNA4-2 d

S.Figure2 1.5 1.5 a 1.0 1.0 * Relative migration Relative migration 0.5 0.5 ** shKPNA4 shKPNA4 Scramble 0 h 24 h Scramnle 0 0 shKPNA4 shKPNA4 Scramble Scramble Scramble Cytoplasm Nuclear KPNA4 b shKPNA4 α-tubulin shKPNA4 Scramble Histone H4 c

S.Figure3 a shKPNA4 Scramble Day 1 Day 2 Day 3 b ns

S.Figure4 a b

S.Figure5 Scramble-miR-NC Scramble-miR-708 ** * ns shKPNA4-miR-NC shKPNA4-miR-708

S.Figure6 PC3 Scramble PC3 shKPNA4 PC3 Scramble PC3 shKPNA4 BLI Cell number (105)

S.Figure 7 Scramble shKPNA4

S.Figure8 a Scramble shKPNA4 shKPNA4 Scramble β-actin TNF-α TNF-β TNF-α/β TNF-α/β b c ** ** ** ** TNF-β TNF-α

S.Figure9 a shKPNA4+TNF-α TNF-α+TNF-β TNF-α TNF-β Control Scramble shKPNA4 b 3 1.5 * ** * ** ** 2 1.0 TRAP+cells TRAP+cells 1 0.5 0 0 Scramble TNF-β Control shKPNA4 TNF-α c d TNF-α+TNF-β shKPNA4+TNF-α Scramble shKPNA4 E

S.Figure10 a Relative fold change b shKPNA4VSScramble

Relative Migration and Function of shKPNA4 in TNF-α/β Signaling Pathway

Relative Migration and Function of shKPNA4 in TNF-α/β Signaling Pathway

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Additional file Figure S.1.

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Wang et al. Figure S 1.

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