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Intermediate Filaments

Intermediate Filaments

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Intermediate Filaments

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Presentation Transcript

  1. Cytokeratin Mitochondria DNA Intermediate Filaments Text and image sources are included using the notes function of this file

  2. The IMF Family form heterodimers

  3. Assembly central rod domain as simple as abcdefg bundles of 8 tetramers

  4. Atomic Model of the Dimer

  5. Cytokeratins in Epithelial Cells Keratins Lamins

  6. Epidermolysis Bullosa Simplex arises from keratin point mutations • Dowling-Meara - widespread blistering • Koebner - blistering confined to hands and feet • Weber-Cockayne - least severe

  7. Stretching an EBS cell destroys the keratin cytoskeleton

  8. Vimentin in Fibroblasts

  9. Normal Skin Squalous cell carcinoma Cancer detection Cytokeratins are normally expresssed in skin but not in the underlying tissues. Antibody staining can reveal hyperplasia and metastasis of epithelial cells into those tissues as a cancer progresses.

  10. NF-H in neurons GFAP in glia Neurofilamentsin Neurons

  11. Active Remodeling (note - short clips are repeated several times in each part of the sequence)

  12. Cytokeratin-GFP dynamics 4 hour sequence

  13. Cytokeratin-GFP dynamics during mitosis 40 minute sequence

  14. Okadaic acid (phosphatase inhibitor) results in cytokeratindisassembly Cytokeratin 13::GFP 200+ minute sequence

  15. Cytokeratin-GFP dynamics require ATP 96 minute sequence

  16. Cytokeratin-GFP dynamics requires microtubules 115 minute sequence

  17. Microinjection of anti-detyrosinated tubulin antibodies collapses the IMF network

  18. Cytokeratin-GFP dynamics doesn’t require micro-filaments 115 minute sequence

  19. Time-lapse Vimentin-GFP Nocodazole collapse Close-up cell edge

  20. Vimentin overlays Distinct from Microfilaments Co-align with Microtubules

  21. Plectin links Vimentin IMFsand Microtubules

  22. The Cytomatrix

  23. Tensegrity F-actin

  24. The Axonal Matrix

  25. Murine Macrophage

  26. Nocodozole-treatment depolymerizes mts and causes lamellipodial retraction