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Characterization of NRF Expression Vectors and Peptide Sequences in Transcriptional Control

This study presents detailed analysis of NRF expression vectors, including pLexA-p65 and pRL-Tk. Using bioluminescence assays, we investigate various peptide sequences related to NRF binding. Figures denote binding interactions, peptide lengths, and specific sequences exhibiting transcriptional activity. The findings deepen the understanding of NRF function in gene regulation, providing insights into peptide interactions and their implications in transcriptional control mechanisms.

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Characterization of NRF Expression Vectors and Peptide Sequences in Transcriptional Control

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  1. NRE pLexA-p65 LexA p65 MPSV pL6NLuc LexA Firefly Luc pRL-Tk Tk Renilla Luc + + + + pL6NLuc - + + + pLexA-p65 -- NRF(1-380) NRF(1-690) NRF expression vectors Figure 1

  2. Figure 2 A p65 bound p50 bound B NRF(1-380)TAP + p65RHD NRF(1-380)TAP + p65 NRF(1-380)TAP + p65RHD NRF(1-380)TAP + p65 NRF(1-380)TAP p65 endogenous p65 p65 RHD bound total

  3. spot peptide sequence 1 1MDELFPLIFPAEPAQ15 24LFPLIFPAEPAQASG18 3 7LIFPAEPAQASGPYV21 410PAEPAQASGPYVEII24 . . Figure 3 A B Control Spots 1 26 51 76 RRL 1 26 51 76 NRF-Strep 85 199 114 Spots 1 26 51 76 309 274 246

  4. peptide length • spot peptide sequence • 1 88HELVGKDC95 • 289ELVGKDCR96 • 3 90LVGKDCRD97 • 87PHELVGKDC95 • 88HELVGKDCR96 • 89ELVGKDCRD97 • 86HPHELVGKDC95 • 87PHELVGKDCR96 • 88HELVGKDCRD97 8 aa 8 aa 8 aa .. 9 aa 9 aa 9 aa .. 10 aa 10 aa 10 aa .. 13 11 14 9 12 15 10 Figure 4 A 81 PPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQ 114 B NRF-Strep peptide length spots 8 8-9 aa 1 9-10 aa 26 10-11 aa 51 11-12 aa 76 12-13 aa 101 13-14 aa 126 14-15 aa 151 15 aa 176

  5. spot peptide sequence 1 199VNRNSGSCLGGDEIF213 2200NRNSGSCLGGDEIFL214 3 201RNSGSCLGGDEIFLL215 . . Figure 5 A 199 VNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHR 246 B NRF-Strep Spots 1 26

  6. spot peptide sequence 1 274RPSDRELSEPMEFQY288 2 274APSDRELSEPMEFQY288 3 274RASDRELSEPMEFQY288 4 274RPADRELSEPMEFQY288 18 277DRELSEPMEFQYLPD291 19 277ARELSEPMEFQYLPD291 20 277DAELSEPMEFQYLPD291 21 277DRALSEPMEFQYLPD291 . . . . NRF-Strep spots 1 26 51 76 101 Figure 6 A 274 RPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTY 306 B

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