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Validation of accelerated c-MYC expression kinetics by real-time PCR in osteogenic hMSCs

This supplemental figure validates the enhanced c-MYC expression through real-time PCR analysis in osteogenic human mesenchymal stem cells (hMSCs) treated with DEX, BMP2, and 1,25(OH)2D3. Data show mean c-MYC expression over time corrected using RPS27A levels. Error bars represent standard deviations.

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Validation of accelerated c-MYC expression kinetics by real-time PCR in osteogenic hMSCs

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  1. SUPPLEMENTAL FIGURE S1 Fig. S1. Validation of accelerated expression kinetics of c-MYC by quantitative real-time RT-PCR. Real-time PCR analysis was performed as previously described [10] using RNA from an independent osteogenic hMSC time-course experiment. Measurements were performed in duplicate, after which the calculated mean c-MYC expression per time point and treatment was corrected for corresponding expression levels of RPS27A, a housekeeping gene. Error bars indicate standard deviations. ○, –DEX ; ●, DEX; □, DEX+BMP2; ■, DEX+1,25(OH)2D3. 61

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