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Supplemental Figure S1

Supplemental Figure S1. IgG. Con. Snail1-Flag. DNA-PKcs. IgG. Snail1-Flag. IgG. Supplemental Fig. S2. Non-neoplastic. Case 1. Case 2. Case 3. Case 4. Case 5. DNA-PKcs. Human colon cancer. Snail1. DNA-PKcs. Adenocarcinoma. Snail1. Human lung cancer. DNA-PKcs.

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Supplemental Figure S1

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  1. Supplemental Figure S1 IgG Con Snail1-Flag DNA-PKcs IgG Snail1-Flag IgG

  2. Supplemental Fig. S2 Non-neoplastic Case 1 Case 2 Case 3 Case 4 Case 5 DNA-PKcs Human colon cancer Snail1 DNA-PKcs Adenocarcinoma Snail1 Human lung cancer DNA-PKcs Squamouse cell carcinoma Snail1

  3. Supplemental Fig. S3 Snail1 GenBank: AF131208.1 1 atgccgcgctctttcctcgtcaggaagccctccgaccccaatcggaagcctaactacagc M P R S F L V R K P S D P N R K P N Y S 61 gagctgcaggactctaatccagagtttaccttccagcagccctacgaccaggcccacctg E L Q D S N P E F T F Q Q P Y D Q A H L 121 ctggcagccatcccacctccggagatcctcaaccccaccgcctcgctgccaatgctcatc L A A I P P P E I L N P T A S L P M L I 181 tgggactctgtcctggcgccccaagcccagccaattgcctgggcctcccttcggctccag W D S V L A P Q A Q P I A W A S L R L Q 241 gagagtcccagggtggcagagctgacctccctgtcagatgaggacagtgggaaaggctcc E S P R V A E L T S L S D E D S G K G S(100) 301 cagccccccagcccaccctcaccggctccttcgtccttctcctctacttcagtctcttcc Q P P S P P S P A P S S F S S T S V S S 361 ttggaggccgaggcctatgctgccttcccaggcttgggccaagtgcccaagcagctggcc L E A E A Y A A F P G L G Q V P K Q L A 421 cagctctctgaggccaaggatctccaggctcgaaaggcctccaactgcaaatactgcaac Q L S E A K D L Q A R K A S N C K Y C N 481 aaggaatacctcagcctgggggcgctgaagatgcac K E Y L S L G A L K M H

  4. Supplemental Fig. S4 a * S100D Snail1 S100A * A549 WT Con b 0 5 10 Snail1 Migration activity (Fold increase) S100A S100D Con WT Snail1 Normal control CT26 cells CT26-WT CT26-S100A CT26-S100D β -Actin * S100D H&E Snail1 Normal Con WT S100A S100D S100A A549 * WT Con 2.5 0.4 0.6 0.8 1 1.2 E-cadherin promoter activity 2 1.5 Lung weight (%) * 1 0.5 0 WT Con S100D S100A Normal CT26

  5. 0 0 0 0 10 10 10 10 30 30 30 30 60 60 60 60 0 0 0 10 10 10 30 30 30 60 60 60 Supplemental Fig. S5 a NCI-H460 NCI-H460 Con Snail1 siRNA : SC Snail1 (IR, min) p-p53(Ser15) p53 g-H2AX Snail1 b-Actin c Snail-Flag b WT S100A S104A/S107A Snail1-Flag Snail1-Flag (IR, min) S104A / S107A S104A / S107A pCR3.1 pCR3.1 S100A p-p53(Ser15) S100A WT WT p53 Snail1 γ-H2AX β-Actin Snail1 NCI-H460 A549 β-Actin pCR3.1 3 WT S100A S104A-107A 1 DNA-PK kinase activity (Fold increase) 0.5 0 - - Snail1 + + + + + + H460 A549

  6. M059J 0 10 90 120 Supplemental Fig. S6 a * 60 * 50 40 Death(%) 30 20 10 0 - + - + Snail1 Si-Snail1 G2/M phase (%) b 100 80 c d 60 Death(%) 40 20 (IR, min) 30 60 0 Con Snail1 p-DNA-PKcs (Ser2056) * M059J DLD-1 * DNA-PKcs Snail1 Snail1/shDNA-PKcs 4500 * p-GSK3β(Tyr216) 4000 * 3500 * GSK3β 3000 β-Actin 2500 2000 1500 1000 500 (day) 0 14 19 26 29 33

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