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Supplemental Figure S1. 0. MOI. 5. 10. 15. 20. 30. pSTAT3. Flag. MDA-MB-231 cells were treated with different doses of adenoviral STAT3 dominant negative.
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Supplemental Figure S1 0 MOI 5 10 15 20 30 pSTAT3 Flag MDA-MB-231 cells were treated with different doses of adenoviral STAT3 dominant negative. The decrease in pSTAT3 was dose-dependant, and the pSTAT3 band became barely detectable at a MOI (multiplicity of infection) of 15. FLAG, which was tagged to the STAT3 dominant negative construct, increased with the MOI’s. Cells infected with adenoviral GFP (negative control) did not show any detectable decrease in pSTAT3 (not shown).
Supplemental Figure S2 Adenoviral STAT3 dominant negative Adenoviral GFP Control 0 0 5 10 15 20 30 5 10 15 20 30 MOI MW (kDa) PARP 116 89 Cleaved PARP Caspase 3 35 19 Cleaved caspase 3 17 MDA-MB-231 cells were infected with adenoviral STAT3 dominant negative at different MOI’s. After 48 hours, apoptosis was assessed by detecting cleavage of caspase 3 and PARP. MDA-MB-231 cells showed no sign of apoptosis after the treatment. MDA-MB-231 cells treated with adenoviral GFP (a negative control) showed no detectable evidence of apoptosis. NIH/3T3 cells treated with 1 µM staurosporine served as a positive control.