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Investigating TGF-β Signaling in MCF7 and SKOV-3 Cell Lines with Par-4 and EMT Markers

This supplementary figure series explores the effects of TGF-β1, TGF-β2, and TGF-β3 on MCF7 and SKOV-3 cell lines, focusing on key proteins involved in epithelial-mesenchymal transition (EMT) such as Snail, E-cadherin, and vimentin. The analysis includes Western blot results showing the expression levels of Par-4, p-Smad2, GAPDH, and other markers. The study aims to elucidate the role of Par-4 in TGF-β signaling pathways and its implications in cancer progression and apoptosis.

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Investigating TGF-β Signaling in MCF7 and SKOV-3 Cell Lines with Par-4 and EMT Markers

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  1. SUPPLEMENTARY FIGURES

  2. Supp. 1 MCF7 SKOV-3 TGF-3 TGF-2 TGF-1 - - - TGF-3 TGF-2 TGF-1 - + - - - + + - - - - - + - - - + - - - + A. Par-4 Par-4 -actin -actin * *** * MCF7 SKOV-3 B. TGF-3 TGF-2 TGF-1 - - - TGF-3 TGF-2 TGF-1 - + - - - + + - - - - - + - - - + - - - + * *** ** C. HIESC KLE Hec-1-A Hela SKOV-3 MCF7 TGF-RI TGF-RII GAPDH

  3. KLE Supp. 2 SKOV-3 C A Hec-1-A Control Alk5 inh. - + Par-4 P-Smad2 GAPDH B Hiesc TGF-3 MCF7 TGF-3 TGF-2 TGF-1 + - - - - - - + - TGF-3 TGF-2 TGF-1 - - + + - - - - - - + - - - + Days 0 3 6 Snail E-cadherin Claudin-1 N-Cadherin D N-cadherin β-actin Vimentin Snail GAPDH β-actin TGF-3 Days 0 3 6 SKOV-3 KLE TGF-3 TGF-2 TGF-1 - - - + - - - + - - - + TGF-3 TGF-2 TGF-1 + - - - - - - + - - - + Par-4 Par-4/GAPDH E GAPDH E-cadherin E-cadherin 5 4 3 2 1 0 N-cadherin N-cadherin Snail Snail β-actin β-actin

  4. Supp. 3 SKOV-3 MCF7 C. A - + + - - + + - Par-4 Par-4 Empty Empty Myc-Par-4 Myc-Par-4 Merge Par-4 Hoechst E-cadherin Claudin-1 Vimentin β-actin Control Snail β-actin TGF-1 B. TGF-1 TGF-2 TGF-3 Control TGF-2 Par-4 p-Smad2 TGF-3 PARP GAPDH C N C N C N C N

  5. Supp. 4 Mitochondria Bax PDK1 Cytochrome C Bak Apaf-1 Bad Bad P Thr308 Bcl-2 P Akt PIP3 P C9 Ser473 XIAP IKK PIP2 Par-4 cleavage C3 PTEN P p110 p65 Par-4 PI3K Ub IkB p85 p50 P XIAP p65 Apoptosis P p50 TGFR IkB TGFR Par-4 EMT XIAP P Smad2/3 TGFβ Smad2/3 Nucleus P TGFR Smad4 TGFR TGFβ

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